- PN: A212197
45x Ab-conjugated beads (S5P3 - Mouse MIP-1β Ab-bead). PN: A212197A. One vial containing 100 µL of anti-mouse MIP-1β conjugated to AimPlex Bead S5P3.
25x Biotin-detection Ab (Mouse MIP-1β Biotin-dAb). PN: A212197B. One vial containing 100 µL of biotinylated anti-mouse MIP-1β.
Lyophilized Standard Mix - Mouse Group 2 Panel B, 8-Plex. PN: MG20008. One vial containing lyophilized recombinant mouse G-CSF, GM-CSF, IL-28A/B, M-CSF, MIP-1α, MIP-1β, OSM, and TSLP. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying human Mouse CCL4/SCYA4/MIP-1β. Can be multiplexed with other analytes in Mouse Group 2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 5 pgmL
LLOQ: < 10 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Human Group 2: Negligible
Sample volume: 15 µL/test
Chemokine (C-C motif) ligand 4 (CCL4), also known as small inducible cytokine A4 (SCYA4) and Macrophage Inflammatory Protein-1 beta (MIP-1β) is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Perforin-low memory CD8+ T cells normally synthesize MIP-1-beta. Diseases associated with CCL4 include stachybotrys chartarum and retinal vasculitis.
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4. Kamin-Lewis R, Abdelwahab SF, Trang C, Baker A, DeVico AL, Gallo RC, Lewis GK (July 2001). "Perforin-low memory CD8+ cells are the predominant T cells in normal humans that synthesize the β-chemokine macrophage inflammatory protein-1β". Proc. Natl. Acad. Sci. U.S.A. 98 (16): 9283–8. doi:10.1073/pnas.161298998. PMC 55412. PMID 11470920.