96 Tests PN: B213435

Components:

45x Ab-conjugated beads (S5P3 - mouse CD23/sCD23/Fcer2 ab-bead). PN: B213435A. One vial containing 100 µL of anti-mouse CD23/sCD23/Fcer2 conjugated to AimPlex Bead S5P3.

25x Biotin-detection Ab (Mouse CD23/sCD23/Fcer2 Biotin-dAb). PN: B213435B. One vial containing 100 µL of biotinylated anti-mouse CD23/sCD23/Fcer2.

Lyophilized Standard - Mouse CD23/sCD23/Fcer2. PN: B213435S. One vial containing lyophilized recombinant mouse CD23/sCD23/Fcer2.  

Application: Optimal antibody pair and antigen standard for assaying mouse CD23/sCD23/Fcer2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, platelet-poor plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 10 pg/mL

  • Quantitation range:

  • LLOQ: < 20 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. The allergen responsible in dust mite allergy Der p 1 is known to cleave CD23 from a cell’s surface. As CD23 is soluble, it can move freely and interact with cells in plasma. CD23 is used in the differentiation of chronic lymphocytic leukemia (CD23-positive) from mantle cell lymphoma (CD23-negative)

References:

1.    Barna G, et al. The cut-off levels of CD23 expression in the differential diagnosis of MCL and CLL. Hematological Oncology, 2008 26 (3): 167–70. doi:10.1002/hon.855. PMID 18381689. S2CID 20572446.

2.    Henningsson F, et al. IgE-mediated enhancement of CD4+ T cell responses in mice requires antigen presentation by CD11c+ cells and not by B cells. PLOS ONE. 6 (7): e21760. Bibcode:2011PLoSO...621760H. doi:10.1371/journal.pone.0021760. PMC 3130775. PMID 21765910.