96 Tests PN: C280005

32 Tests PN: C280005T

Components:

1x Ab-conjugated beads (premixed).

  • 96 Tests PN: C280005A. One bottle containing 4.7 mL of the following AimPlex antibody-bead conjugates

  • 32 Tests PN: C280005TA. One bottle containing 1.5 mL of the following AimPlex antibody-bead conjugates

    • S4P2 - anti-mouse IL-2

    • S4P8 - anti-mouse IFNγ

    • S5P2 - anti-mouse IL-4

    • S5P7 - anti-mouse IL-5

    • S5P11 - anti-mouse TNFα

2x Biotin-detection Ab (premixed).

  • 96 Tests PN: C280005B. One vial containing 1.25 mL of the following biotinylated antibodies.

  • 32 Tests PN: C280005TB. One vial containing 0.45 mL of the following biotinylated antibodies.

    • Biotin - anti-mouse IL-2

    • Biotin - anti-mouse IFNγ

    • Biotin - anti-mouse IL-4

    • Biotin - anti-mouse IL-5

    • Biotin - anti-mouse TNFα

2x Mouse/Rat dAb Diluent.

  • 96 Tests PN: 07MRDX21. One vial containing 1.25 mL of biotin detection antibody diluent.

  • 32 Tests PN: 07MRDX21T. One vial containing 0.45 mL of biotin detection antibody diluent.

Lyophilized Standard Mix- Mouse Th1/Th2 5-plex.

  • 96 & 32 Tests PN: MG28005. One vial containing lyophilized recombinant IFNγ, IL-2, IL-4, IL-5, and TNFα.

      Standard Info Sheet: Indicates reconstitute volume and standard concentrations.

Basic Kit for Premixed Panels: 

  • 1x Streptavidin-PE (SAPE). 

    • 96 Tests PN: 07SP0011. One bottle containing 2.5 mL of 1x SAPE.

    • 32 Tests PN: 07SP0011T. One bottle containing 1 mL of 1x SAPE.

  • 10x Reading Buffer. 

    • 96 Tests PN: 07RB1. One bottle containing 4 mL of 10x buffer.

    • 32 Tests PN: 07RB1T. One bottle containing 1.5 mL of 10x buffer.

  • 10x Wash Buffer. 

    • 96 Tests PN: 07WB1. One vial containing 15 mL of 10x buffer.

    • 32 Tests PN: 07WB1T. One vial containing 5 mL of 10x buffer.

  • PCR 8-tube Strip. 

    • 96 & 32 Tests PN: 07P8T1. 2 strips.

  • Filter Plate and Lid. 

    • 96 & 32 Tests PN: 07FPL1. 1 96- well filter plate and 1 plate lid.

  • Plate Seals. 

    • PN: 07PSR1. 96 Tests: 4 sealers. 32 Tests: 3 sealers.

Application: Optimal antibody pairs and antigen standards for assaying the mouse Th1/Th2 5-Plex Panel.  To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 96 Tests (PN: U100005), 32 Tests (PN: U100005T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage: 2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LODs): IFNγ, < 0.5 pg/mL; IL-2, < 2 pg/mL; IL-4, < 0.5 pg/mL; IL-5, < 5 pg/mL; TNFα, < 0.5 pg/mL.

  • LLOQs: IFNγ, < 2 pg/mL; IL-2, < 5 pg/mL; IL-4, < 2 pg/mL; IL-5, < 10 pg/mL; TNFα, < 2 pg/mL.

  • ULOQs: IFNγ, > 5,000 pg/mL; IL-2, > 5,000 pg/mL; IL-4, > 1,500 pg/mL; IL-5, > 5,000 pg/mL; TNFα, > 1,500 pg/mL.

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in the Panel: Negligible

  • Sample volume: 15 µL/test

 For Research Use Only.  Not for use in diagnostic procedures.