96 Tests PN: A213209
45x Ab-conjugated beads (S4P3 - Mouse sP-selectin Ab-bead). PN: A213209A. One vial containing 100 µL of anti-mouse sP-selectin conjugated to AimPlex Bead S4P3.
25x Biotin-detection Ab (Mouse sP-selectin Biotin-dAb). PN: A213209B. One vial containing 100 µL of biotinylated anti-mouse sP-selectin.
Lyophilized Standard Mix - Mouse Group 5 Panel A, 9-Plex. PN: MG5009A. One vial containing lyophilized recombinant sCD10, sCD14, sICAM, sE-selectin, sL-selectin, sP-selectin, sVCAM, sCD30L, and sCD40L. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
Application: Optimal antibody pair and antigen standard for assaying human Mouse P-selectin/sLECAM-3/sCD62P. Can be multiplexed with other analytes in Mouse Group 5. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 50 pg/mL
LLOQ: < 100 pg/mL
ULOQ: > 10,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Mouse Group 5: Negligible
Sample volume: 15 µL/test
P-selectin is a protein that functions as a cell adhesion molecule (CAM) on the surfaces of activated endothelial cells, which line the inner surface of blood vessels, and activated platelets. In un-activated endothelial cells, it is stored in granules called Weibel-Palade bodies. In un-activated platelets P-selectin is stored in α-granules. Other names for P-selectin include Leukocyte-Endothelial Cell Adhesion Molecule 3 (LECAM3), CD62P, and Granule Membrane Protein 140 (GMP-140). This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Diseases associated with SELP include intermittent claudication and arteriosclerosis obliterans.
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Pan J, Xia L, McEver RP (April 1998). "Comparison of promoters for the murine and human P-selectin genes suggests species-specific and conserved mechanisms for transcriptional regulation in endothelial cells". J. Biol. Chem. 273 (16): 10058–67. doi:10.1074/jbc.273.16.10058. PMID 9545353.