• PN: B213362

COMPONENTS

45x Ab-conjugated beads (S5P6 - Mouse sTWEAK-R Ab-bead). PN: B213362A. One vial containing 100 µL of anti-mouse sTWEAK-R conjugated to AimPlex Bead S5P6.

25x Biotin-detection Ab (Mouse sTWEAK-R Biotin-dAb). PN: B213362B. One vial containing 100 µL of biotinylated anti-mouse sTWEAK-R.

Lyophilized Standard Mix - Mouse Group 5 Panel B, 10-Plex. PN: MG5010B. One vial containing lyophilized recombinant sCD27, sCD105, sIFNγR1, sTNFR1, sTNFRII,  sTWEAK-R, sVEGFR1, sVEGFR2, sIL-1R1, and sRAGE.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Mouse TNFRSF12a/sCD266/sTWEAK-R. Can be multiplexed with other analytes in Mouse Group 5.  To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 50 pg/mL

Quantitation range:

LLOQ: < 100 pg/mL

ULOQ: > 20,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 5: Negligible

Sample volume: 15 µL/test

Description:

Tumor necrosis factor receptor superfamily member 12A also known as the Tweak-receptor (TweakR) and CD 266 is a protein that in humans is encoded by the TNFRSF12A gene. NFAT1 regulates the expression of TWEAKR and its ligand TWEAK with lipocalin 2 to increase breast cancer cell invasion. Diseases associated with TNFRSF12A include glioblastoma multiforme and glioblastoma.

References:

1.      Feng SL, Guo Y, Factor VM, Thorgeirsson SS, Bell DW, Testa JR, Peifley KA, Winkles JA (Jun 2000). "The Fn14 Immediate-Early Response Gene Is Induced During Liver Regeneration and Highly Expressed in Both Human and Murine Hepatocellular Carcinomas". Am J Pathol 156 (4): 1253–61. doi:10.1016/S0002-9440(10)64996-6. PMC 1876890. PMID 10751351.

2.      Meighan-Mantha RL, Hsu DK, Guo Y, Brown SA, Feng SL, Peifley KA, Alberts GF, Copeland NG, Gilbert DJ, Jenkins NA, Richards CM, Winkles JA (Jan 2000). "The mitogen-inducible Fn14 gene encodes a type I transmembrane protein that modulates fibroblast adhesion and migration". J Biol Chem 274 (46): 33166–76. doi:10.1074/jbc.274.46.33166. PMID 10551889.

3.      Gaudineau B, Fougère M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S (October 2012). "Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion". J. Cell. Sci. 125 (Pt 19): 4475–86. doi:10.1242/jcs.099879. PMID 22767506.