• PN: B213449

COMPONENTS

45x Ab-conjugated beads (S5P12 - Mouse sVEGFR1 Ab-bead). PN: B213449A. One vial containing 100 µL of anti-mouse sVEGFR1 conjugated to AimPlex Bead S5P12.

25x Biotin-detection Ab (Mouse sVEGFR1 Biotin-dAb). PN: B213449B. One vial containing 100 µL of biotinylated anti-mouse sVEGFR1.

Lyophilized Standard Mix - Mouse Group 5 Panel B, 10-Plex. PN: MG5010B. One vial containing lyophilized recombinant sCD27, sCD105, sIFNγR1, sTNFR1, sTNFRII, sIL-1R1, sTWEAK-R, sVEGFR1, sVEGFR2, and sRAGE.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying human Mouse VEGFR1/sFlt-1/sVEGFR1. Can be multiplexed with other analytes in Mouse Group 5.  To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 20 pg/mL

Quantitation range:

LLOQ: < 40 pg/mL

ULOQ: > 10,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Human Group 5: Negligible

Sample volume: 15 µL/test

Description:

Vascular endothelial growth factor receptor 1 (VEGFR1), also known as Flt-1, is an oncogene. Flt-1 belongs to the src gene family and is related to oncogene ROS (MIM 165020). Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The sequence structure of the FLT gene resembles that of the FMS gene (MIM 164770); hence the name FLT is an acronym for FMS-like tyrosine kinase. Diseases associated with Flt1 include microcystic meningioma and eclampsia.

References:

1.      Shibuya M, Yamaguchi S, Yamane A, Ikeda T, Tojo A, Matsushime H, Sato M (Apr 1990). "Nucleotide sequence and expression of a novel human receptor-type tyrosine kinase gene (flt) closely related to the fms family". Oncogene 5 (4): 519–24. PMID 2158038.

2.      Cunningham SA, Arrate MP, Brock TA, Waxham MN (Nov 1997). "Interactions of FLT-1 and KDR with phospholipase C gamma: identification of the phosphotyrosine binding sites". Biochemical and Biophysical Research Communications 240 (3): 635–9. doi:10.1006/bbrc.1997.7719. PMID 9398617.

3.      Olofsson B, Korpelainen E, Pepper MS, Mandriota SJ, Aase K, Kumar V, Gunji Y, Jeltsch MM, Shibuya M, Alitalo K, Eriksson U (Sep 1998). "Vascular endothelial growth factor B (VEGF-B) binds to VEGF receptor-1 and regulates plasminogen activator activity in endothelial cells". Proceedings of the National Academy of Sciences of the United States of America 95 (20): 11709–14. doi:10.1073/pnas.95.20.11709. PMC 21705. PMID 9751730.

4.      Makinen T, Olofsson B, Karpanen T, Hellman U, Soker S, Klagsbrun M, Eriksson U, Alitalo K (Jul 1999). "Differential binding of vascular endothelial growth factor B splice and proteolytic isoforms to neuropilin-1". The Journal of Biological Chemistry 274 (30): 21217–22. doi:10.1074/jbc.274.30.21217. PMID 10409677.