PN: B412245

COMPONENTS

45x Ab-conjugated beads (S4P5 - NHP SDF-1 Ab-bead). PN: B412245A. One vial containing 100 µL of anti-NHP SDF-1 conjugated to AimPlex Bead S4P5.

25x Biotin-detection Ab (NHP SDF-1 Biotin-dAb). PN: B412245B. One vial containing 100 µL of biotinylated anti-NHP SDF-1.

Lyophilized Standard - NHP SDF-1. PN: B412245S. One vial containing lyophilized recombinant SDF-1.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying NHP CXCL12/SDF-1.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

NOTE: SDF-1 binds to CXCR4 with high affinity.  CXCR4 is expressed on platelets.  Sample types containing low levels of platelets such as platelet-poor plasma are recommended. 

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, platelet-poor plasma,, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

The stromal cell-derived factor 1 (SDF-1) also known as C-X-C motif chemokine 12 (CXCL12 is a small cytokine that belongs to the chemokine family, members of which activate leukocytes and are often induced by pro-inflammatory stimuli such as lipopolysaccharide, TNF, or IL1.  CXCL12 is strongly chemotactic for lymphocytes. During embryogenesis it directs the migration of hematopoietic cells from fetal liver to bone marrow and the formation of large blood vessels. In adulthood, CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) from the bone marrow through a CXCR4 dependent mechanism. It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumor progression. CXCL12 also has a role in tumor metastasis where cancer cells that express the receptor CXCR4 are attracted to metastasis target tissues that release the ligand, CXCL12. In breast cancer, however, increased expression of CXCL12 determines a reduced risk of distant metastasis. CXCL12 is shown to be responsible for recruiting macrophages to breast tumors in mice in response to the experimental anti-cancer drug combretastatin A-4 phosphate, which damages tumor blood vessels. Diseases associated with CXCL12 include Retinal Hemangioblastoma and Whim Syndrome.

References:

1.      De La Luz Sierra M, Yang F, Narazaki M, Salvucci O, Davis D, Yarchoan R, Zhang HH, Fales H, Tosato G (Apr 2004). "Differential processing of stromal-derived factor-1alpha and stromal-derived factor-1beta explains functional diversity". Blood 103 (7): 2452–9. doi:10.1182/blood-2003-08-2857. PMID 14525775.

2.     Bleul CC, Fuhlbrigge RC, Casasnovas JM, Aiuti A, Springer TA (Sep 1996). "A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1)". The Journal of Experimental Medicine 184 (3): 1101–9. doi:10.1084/jem.184.3.1101. PMC 2192798. PMID 9064327.

3.      Ara T, Nakamura Y, Egawa T, Sugiyama T, Abe K, Kishimoto T, Matsui Y, Nagasawa T (Apr 2003). "Impaired colonization of the gonads by primordial germ cells in mice lacking a chemokine, stromal cell-derived factor-1 (SDF-1)". Proceedings of the National Academy of Sciences of the United States of America 100 (9): 5319–23. doi:10.1073/pnas.0730719100. PMC 154343. PMID 12684531.