PN: A412220

COMPONENTS

45x Ab-conjugated beads (S5P12 - NHP TGFalpha Ab-bead). PN: B412220A. One vial containing 100 µL of anti-NHP TGFalpha conjugated to AimPlex Bead S5P12.

25x Biotin-detection Ab (NHP TGFalpha Biotin-dAb). PN: B412220B. One vial containing 100 µL of biotinylated anti-NHP TGFalpha.

Lyophilized Standard - NHP TGFalpha. PN: B412220S. One vial containing lyophilized recombinant TGFalpha.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying NHP TGFalpha.  To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 10 pg/mL

Quantitation range:

LLOQ: < 20 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Sample volume: 15 µL/test

Description:

Transforming growth factor alpha (TGFA) is a protein that is a member of the epidermal growth factor (EGF) family; TGF-α is a mitogenic polypeptide. The protein becomes activated when binding to receptors capable of protein kinase activity for cellular signaling. TGF-α is a transforming growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. TGF-α may act as either a transmembrane-bound ligand or a soluble ligand. It has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. TGF-α can be produced in macrophages, brain cells, and keratinocytes. TGF-α induces epithelial development. Cell proliferation events that involve TGF-α bound to EGFR include wound healing and embryogenesis. TGF-α is also involved in tumerogenesis and believed to promote angiogenesis. TGFα has also been shown to stimulate neural cell proliferation in the adult injured brain. Diseases associated with TGFA include bronchiolo-alveolar adenocarcinoma and bone marrow necrosis.

References:

1.      Ojeda, S.; Ma, Y.; Rage, F. The transforming growth factor alpha gene family is involved in the neuroendocrine control of mammalian puberty. Mol. Psychiatry 1997, 2, 355.

2.      Ferrer, I.; Alcantara, S.; Ballabriga, J.; Olive, M.; Blanco, R.; Rivera, R.; Carmona, M.; Berruezo, M.; Pitarch, S.; Planas, A. Transforming growth factor- α (TGF-α) and epidermal growth factor-receptor (EGF-R) immunoreactivity in normal and pathologic brain. Prog. Neurobiol. 1996, 49, 99.

3.      Coffey, R.; Gangarosa, L.; Damstrup, L.; Dempsey, P. Basic actions of transforming growth factor- α and related peptides. Eur. J. Gastroen. Hepat. 1995, 7, 923.

4.      McInnes, C; Wang, J; Al Moustafa, AE; Yansouni, C; O'Connor-McCourt, M; Sykes, BD (1998). "Structure-based minimization of transforming growth factor-alpha (TGF-alpha) through NMR analysis of the receptor-bound ligand. Design, solution structure, and activity of TGF-alpha 8-50".". J. Biol. Chem 273 (42): 27357–63. doi:10.1074/jbc.273.42.27357.