96 Tests PN: B411130
45x Ab-conjugated beads (S4P12 - NHP IL-12p70 ab-bead). PN: B411130A. One vial containing 100 µL of anti-NHP IL-12p70 conjugated to AimPlex Bead S4P12.
25x Biotin-detection Ab (NHP IL-12p70 Biotin-dAb). PN: B411130B. One vial containing 100 µL of biotinylated anti-NHP IL-12p70.
Lyophilized Standard Mix-NHP Group 1 Panel B. PN: NG10011. One vial containing lyophilized recombinant Eotaxin, IL-1β, IL-1RA, IL-8, IL-12p70, IP-10, MCP-1, MCP-3, RANTES. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
Application: Optimal antibody pair and antigen standard for assaying NHP IL-12p70. Can be multiplexed with other analytes in NHP Group 1. To be used in conjunction with the AimPlex NR Basic Kit (PN: P100001) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
Storage: 2-8 C in the dark.
Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 3 pg/mL
LLOQ: < 10 pg/mL
ULOQ: > 2,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in NHP Group 1: Negligible
Sample volume: 15 µL/test
Interleukin 12 has two subunits, IL-12p35 (Accession P48091) and IL-12p40 (Accession P48095) . Represented in its full name, IL-12p70 has a total molecular weight of 70kDa. IL-12 is a T cell stimulating factor and aids in naïve T cell differentiation into Th1 cells. It also mediates NK cells and CD8+ T cells. IL-12, by increasing production of IFN-γ which in turn increases production of IP-10 exerts anti-angiogenic properties. IL-23 is closely related to IL-12 in structure as it shares the p40 subunit. IL-12 is linked to autoimmune diseases such as psoriasis and inflammatory bowel disease though it may inhibit food allergy susceptibility. It also plays an integral role in the immunity against M. tuberculosis.
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Hsieh CS, Macatonia SE, Tripp CS, Wolf SD, O’Garra A, Murphy KM. Development of TH1 CD4+ T cells through IL-12 produced by Listeria-induced macrophages. Science 1993; 260(5107) 547-9. Doi:10.1126/science.8097338.
Hunter CA. New IL-12-family members:IL-23 and IL-27, cytokines with divergent functions. Nature Reviews Immunology. 2005; 5: 521-531. Doi: 10.1038/nri1648.
Robinson CM, Nau GJ. Interleukin-12 and interleukin-27 regulate macrophage control of Mycobacterium tuberculosis. J Infect Dis. 2008; 198(3): 359-366. Doi:10.1086/589774.
Temblay JN, Bertelli E, Arques JL, Regoi M, Niocletti C. Production of IL-12 by Peyer patch-dendritic cells is critical for the resistance to food allergy. J Allergy Clin Immunol. 2007; 120(3): 659-65. Doi:10.1016/j.jaci.2007.04.044.