96 Tests PN: B313275

Components:

45x Ab-conjugated beads (S5P12 - Rat CD62P/P-selectin/ LECAM-3 Ab-bead). PN: B313275A. One vial containing 100 µL of anti-rat CD62P/P-selectin/ LECAM-3 conjugated to AimPlex Bead S5P12.

25x Biotin-detection Ab (Rat CD62P/P-selectin/ LECAM-3 Biotin-dAb). PN: BB313275B. One vial containing 100 µL of biotinylated anti-rat CD62P/P-selectin/ LECAM-3.

Lyophilized Standard Mix - Rat CD62P/P-selectin/ LECAM-3, PN: BB313275S. One vial containing lyophilized CD62P/P-selectin/ LECAM-3.

Application: Optimal antibody pair and antigen standard for assaying Rat CD62P/P-selectin/ LECAM-3. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 50 pg/mL

  • Quantitation range:

  • LLOQ: < 100 pg/mL

  • ULOQ: > 20,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Sample volume: 15 µL/test

Description:

P-selectin is a protein that functions as a cell adhesion molecule (CAM) on the surfaces of activated endothelial cells, which line the inner surface of blood vessels, and activated platelets. In un-activated endothelial cells, it is stored in granules called Weibel-Palade bodies. In un-activated platelets P-selectin is stored in α-granules. Other names for P-selectin include Leukocyte-Endothelial Cell Adhesion Molecule 3 (LECAM3), CD62P, and Granule Membrane Protein 140 (GMP-140). This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Diseases associated with SELP include intermittent claudication and arteriosclerosis obliterans.

References:

  1. Ryan US, Worthington RE (February 1992). "Cell-cell contact mechanisms". Curr. Opin. Immunol. 4 (1): 33–7. doi:10.1016/0952-7915(92)90120-4. PMID 1375831.

  2. McEver RP, Beckstead JH, Moore KL, Marshall-Carlson L, Bainton DF (July 1989). "GMP-140, a platelet alpha-granule membrane protein, is also synthesized by vascular endothelial cells and is localized in Weibel-Palade bodies". J. Clin. Invest. 84 (1): 92–9. doi:10.1172/JCI114175. PMC 303957. PMID 2472431.

  3. Herrmann SM, Ricard S, Nicaud V, Mallet C, Evans A, Ruidavets JB, Arveiler D, Luc G, Cambien F (August 1998). "The P-selectin gene is highly polymorphic: reduced frequency of the Pro715 allele carriers in patients with myocardial infarction". Hum. Mol. Genet. 7 (8): 1277–84. doi:10.1093/hmg/7.8.1277. PMID 9668170.

  4. Pan J, Xia L, McEver RP (April 1998). "Comparison of promoters for the murine and human P-selectin genes suggests species-specific and conserved mechanisms for transcriptional regulation in endothelial cells". J. Biol. Chem. 273 (16): 10058–67. doi:10.1074/jbc.273.16.10058. PMID 9545353.