96 Tests PN: A311101


45x Ab-conjugated beads (S4P9 - rat IFNγ ab-bead). PN: A311101A. One vial containing 100 µL of anti-rat IFNγ conjugated to AimPlex Bead S4P9.

25x Biotin-detection Ab (Rat IFNγ Biotin-dAb). PN: A311101B. One vial containing 100 µL of biotinylated anti-rat IFNγ.

Lyophilized Standard Mix - Rat Group 1 Panel A, 7-Plex. PN: RG10007. One vial containing lyophilized recombinant rat IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNFα.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

Application: Optimal antibody pair and antigen standard for assaying Rat IFNγ. Can be multiplexed with other analytes in Rat Group 1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

For Research Use Only. Not for use in diagnostic procedures.

Assay Specifications:

  • Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

  • Sensitivity (LOD): < 2 pg/mL

  • Quantitation range:

  • LLOQ: < 5 pg/mL

  • ULOQ: > 5,000 pg/mL

  • Standard dose recovery: 70-130%

  • Intra-assay CV: < 10%

  • Inter-assay CV: < 20%

  • Cross-reactivity of analytes in Rat Group 1: Negligible

  • Sample volume: 15 µL/test


Interferon-gamma (Accession P01581) also known as immune interferon is produced by a variety of leukocytes, most notably T-cells and NK cells. IFN-γ is involved in nearly every phase of immune and inflammatory responses. IFN-γ assists in anti-tumor responses and more generally, in inter-cellular communication for both innate and adaptive immune responses. Interferon-gamma promotes development and activation of Th1 cells, Ig switching in B-cells, and activation of macrophages.

In mice and humans, interferon gamma has been linked to many autoimmune diseases such as non-obese diabetes, lupus nephritis, and experimental autoimmune thyroiditis. There is research to suggest that IFN-γ not only promotes inflammation but may also limit inflammation in certain circumstances by exhibiting anti-proliferative and apoptotic effects. IFN-γ has been used in experimental studies for treating multiple sclerosis, but it appears to have exacerbated the condition.


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  2. Panitch HS, Hirsch RL, Schindler J, Johnson KP. Treatment of multiple sclerosis with gamma interferon Exacerbations associated with activation of the immune system. AAN. 1987; 37 (7). Doi: 10.1212/WNL.37.7.1097.

  3. Pisa P, Halapi E, Pisa EK, Gerdin E, Hising C, Bucht A, Gerdin B, Kiessling R. Selective expression of interleuking 10, interferon gamma, and granulocyte-macrophage colongy-stimulating factor in ovarian cancer biopsies. PNAS. 1992; 89 (16) 7708-7712; doi:10.1073/pnas.89.16.7708.

  4. Tau G, Rothman P. Biologic functions of the IFN-gamma receptors. Allergy. 1999; 54(12):1233–1251.

  5. Zaidi MR, Merlino G. The Two Faces of Interferon-γ in Cancer. Clinical Cancer Research. 2011; 17 (19): 6118-6124. Doi:10.1158/1078-0423.CCR-11-0482.