• PN: B311109

COMPONENTS

45x Ab-conjugated beads (S5P10 - rat IL-10 ab-bead). PN: A311109A. One vial containing 100 µL of anti-rat IL-10 conjugated to AimPlex Bead S5P10.

25x Biotin-detection Ab (Rat IL-10 Biotin-dAb). PN: A311109B. One vial containing 100 µL of biotinylated anti-rat IL-10.

Lyophilized Standard Mix - Rat Group 1 Panel A, 7-Plex. PN: RG10007. One vial containing lyophilized recombinant rat IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNFα.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying Rat IL-10/CSIF. Can be multiplexed with other analytes in Rat Group 1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Rat Group 1: Negligible

Sample volume: 15 µL/test

Description:

IL-12 is a cytokine composed by two different subunits: IL-12p35 (MW 35 kDa) + IL-12p40 (MW 40 kDa). So, the entire cytokine is considered IL-12p70, its total MW is around 70 kDa. IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a co-receptor, CD30, which is associated with IL-12 activity.  IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.

References:

1.      Eskdale J, Kube D, Tesch H, Gallagher G (1997). "Mapping of the human IL10 gene and further characterization of the 5' flanking sequence". Immunogenetics 46 (2): 120–8. doi:10.1007/s002510050250. PMID 9162098.

2.      Mosser DM, Zhang X (Dec 2008). "Interleukin-10: new perspectives on an old cytokine". Immunological Reviews 226 (1): 205–18. doi:10.1111/j.1600-065X.2008.00706.x. PMC 2724982. PMID 19161426.

3.      Zdanov A, Schalk-Hihi C, Gustchina A, Tsang M, Weatherbee J, Wlodawer A (Jun 1995). "Crystal structure of interleukin-10 reveals the functional dimer with an unexpected topological similarity to interferon gamma". Structure 3 (6): 591–601. doi:10.1016/S0969-2126(01)00193-9. PMID 8590020.