• PN: B311178

COMPONENTS

45x Ab-conjugated beads (S5P9 - Rat IL-15 Ab-bead). PN: B311178A. One vial containing 100 µL of anti-rat IL-15 conjugated to AimPlex Bead S5P9.

25x Biotin-detection Ab (Rat IL-15 Biotin-dAb).  PN: B311178B. One vial containing 100 µL of biotinylated anti-rat IL-15.

Lyophilized Standard Mix - Rat Group 2 Panel A, 6-Plex. PN: RG20006. One vial containing lyophilized recombinant rat RANKL, IL-12p40, IL-15, Leptin, NGFb, and VEGF.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying Rat IL-12p40/IL-23p40 . Can be multiplexed with other analytes in Rat Group 2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Rat Group 2: Negligible

Sample volume: 15 µL/test

Description:

Interleukin 15 (IL-15) is a cytokine that binds to and signals through a complex composed of IL-2/IL-15 receptor beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by viruses. IL-15 regulates T and NK cell activation and proliferation. Survival signals that maintain memory T cells in the absence of antigen are provided by IL-15. This cytokine is also implicated in NK cell development. In rodent lymphocytes, IL-15 prevents apoptosis by inducing an apoptosis inhibitor, BCL2L1/BCL-x(L).  In humans with celiac disease IL-15 similarly suppresses apoptosis in T-lymphocytes by inducing Bcl-2 and/or Bcl-xL.

References:

1.      Steel JC, Waldmann TA, Morris JC (January 2012). "Interleukin-15 biology and its therapeutic implications in cancer". Trends Pharmacol. Sci. 33 (1): 35–41. doi:10.1016/j.tips.2011.09.004. PMC 3327885. PMID 22032984.

2.      Di Sabatino A, Calarota SA, Vidali F, Macdonald TT, Corazza GR (February 2011). "Role of IL-15 in immune-mediated and infectious diseases". Cytokine Growth Factor Rev. 22 (1): 19–33. doi:10.1016/j.cytogfr.2010.09.003. PMID 21074481.

3.      Grabstein KH, Eisenman J, Shanebeck K, Rauch C, Srinivasan S, Fung V, Beers C, Richardson J, Schoenborn MA, Ahdieh M (May 1994). "Cloning of a T cell growth factor that interacts with the beta chain of the interleukin-2 receptor". Science 264 (5161): 965–8. doi:10.1126/science.8178155. PMID 8178155.

4.      Lodolce JP, Burkett PR, Koka RM, Boone DL, Ma A (December 2002). "Regulation of lymphoid homeostasis by interleukin-15". Cytokine Growth Factor Rev. 13 (6): 429–39. doi:10.1016/S1359-6101(02)00029-1. PMID 12401478.