• PN: B312229

COMPONENTS

45x Ab-conjugated beads (S4P10 - Rat IP-10 Ab-bead). PN: B312229A. One vial containing 100 µL of anti-rat IP-10 conjugated to AimPlex Bead S4P10.

25x Biotin-detection Ab (Rat IP-10 Biotin-dAb). PN: B312229B. One vial containing 100 µL of biotinylated anti-rat IP-10.

Lyophilized Standard Mix - Rat Group 1 Panel B, 12-Plex. PN: RG10012. One vial containing lyophilized recombinant rat Eotaxin, G-CSF, GM-CSF, IL-1α, IL-1β, IL-5, IL-12p70, IL-13, KC, IP-10, MIP-1α, and MIP-2.  Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pair and antigen standard for assaying Rat IP-10/CXCL10. Can be multiplexed with other analytes in Rat Group 1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

For Research Use Only.  Not for use in diagnostic procedures.

Assay Specifications:

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LOD): < 5 pg/mL

Quantitation range:

LLOQ: < 10 pg/mL

ULOQ: > 5,000 pg/mL

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in Rat Group 1: Negligible

Sample volume: 15 µL/test

Description:

C-X-C Motif Chemokine 10 or Interferon gamma-Induced Protein 10 (Accession P48973) is a member of the CXC chemokine family. CXCL10 is secreted by several tissue and cell types as a reaction to IFN-γ and binds to CXCR3. CXCL10 may act as a cell proliferator or anti-proliferator, depending on which cell-type is being acted upon and as a promoter or inhibitor of angiogenesis, depending on a presence of the ELR motif. CXCL10 induces apoptosis in infectious diseases, but also may contribute to apoptosis during stages of development in the nervous system.  CXCL10 as a transmembrane and mediates in both adaptive and innate immunity.

CXCL10 expression is recognized as a possible biomarker for many diseases such as psoriasis, MS, atherosclerosis, transplant rejection, and inflammatory bowel diseases.

References:

1.     Liu M, Guo S, Hibbert JM, et al. CXCL10/IP-10 in infectious diseases pathogenesis and potential therapeutic implications. Cytokine Growth Factor Rev. 2011;22(3):121–130. doi:10.1016/j.cytogfr.2011.06.001.

2.     Dufour JH, Dziejman M, Liu MT, Leung JH, Lane TE, Luster AD. IFN-γ-Inducible Protein 10 (IP-10; CXCL10)-Deficient Mice Reveal a Role for IP-10 in Effector T Cell Generation and Trafficking. J Immunol. 2002; 168(7): 3195-3204. Doi: 10.4049/jimmunol.168.7.3195.

3.     Vazirinejad R, Ahmadi Z, Arababadi KM, Hassanshahi G, Kennedy D. The Biological Functions, Structure and Sources of CXCL10 and Its Outstanding Part in the Pathophysiology of Multiple Sclerosis. Neuroimmunomodulation. 2014; 21:322-330. Doi: 10.1159/000357780.