- PN: B312233
45x Ab-conjugated beads (S4P4 - Rat MCP-3 Ab-bead). PN: B312233A. One vial containing 100 µL of anti-rat MCP-3 conjugated to AimPlex Bead S4P4.
25x Biotin-detection Ab (Rat MCP-3 Biotin-dAb). PN: B312233B. One vial containing 100 µL of biotinylated anti-rat MCP-3.
Lyophilized Standard Mix - Rat Group 2 Panel B, 7-Plex. PN: RG20007. One vial containing lyophilized recombinant rat LIX, MCP-1, MCP-3, RANTES, SDF-1, ICAM-1, and VCAM-1. Note: If multiple analyte kits on the above target list are ordered as a panel, only one vial of standard mix is supplied for those analyte kits.
STORAGE: 2-8 C in the dark.
IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.
APPLICATION: Optimal antibody pair and antigen standard for assaying Rat CCL7/MARC/MCP-3. Can be multiplexed with other analytes in Rat Group 2. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit (PN: P200201) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.
For Research Use Only. Not for use in diagnostic procedures.
Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD): < 2 pg/mL
LLOQ: < 5 pg/mL
ULOQ: > 5,000 pg/mL
Standard dose recovery: 70-130%
Intra-assay CV: < 10%
Inter-assay CV: < 20%
Cross-reactivity of analytes in Rat Group 2: Negligible
Sample volume: 15 µL/test
Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP-3), small inducible cytokine A7 (SYCA7) and MARC. CCL7 specifically attracts monocytes, and regulates macrophage function. It is produced by certain tumor cell lines and by macrophages. Diseases associated with CCL7 include demyelinating disease and nephrogenic systemic fibrosis.
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2. Opdenakker G, Fiten P, Nys G, Froyen G, Van Roy N, Speleman F, Laureys G, Van Damme J (1994). "The human MCP-3 gene (SCYA7): cloning, sequence analysis, and assignment to the C-C chemokine gene cluster on chromosome 17q11.2-q12". Genomics 21 (2): 403–8. doi:10.1006/geno.1994.1283. PMID 7916328.
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