Rat Th1/Th2/Th17 7-Plex (96 & 32 Tests)

96 Tests PN: C371107

32 Tests PN: C371107T

Components:

1x Ab-conjugated beads (premixed). 

• 96 Tests PN: C371107A. One bottle containing 4.7 mL of the following AimPlex antibody-bead conjugates

• 32 Tests PN: C371107TA. One bottle containing 1.5 mL of the following AimPlex antibody-bead conjugates

  • S4P4 - anti-rat IL-4

  • S4P6 - anti-rat IL-6

  • S4P9 - anti-rat IFNγ

  • S4P12 - anti-rat IL-2

  • S5P5 - anti-rat TNFα

  • S5P7 - anti-rat IL-17A

  • S5P10 - anti-rat IL-10

2x Biotin-detection Ab (premixed). 

• 96 Tests PN: C371107B. One vial containing 1.25 mL of the following biotinylated antibodies.

• 32 Tests PN: C371107TB. One vial containing 0.45 mL of the following biotinylated antibodies.

  • Biotin - anti-rat IL-4

  • Biotin - anti-rat IL-6

  • Biotin - anti-rat IFNγ

  • Biotin - anti-rat IL-2

  • Biotin - anti-rat TNFα

  • Biotin - anti-rat IL-17A

  • Biotin - anti-rat IL-10

2x Mouse/Rat dAb Diluent.

• 96 Tests PN: 07MRDX21T. One vial containing 1.25 mL of biotin detection antibody diluent.

• 32 Tests PN: 07MRDX21T. One vial containing 0.45 mL of biotin detection antibody diluent

Lyophilized Standard Mix - Rat Group 1 Panel A, 7-Plex. 

96 & 32 Tests PN: RG10007. One vial containing lyophilized recombinant rat IFNγ, IL-2, IL-4, IL-6, IL-10, IL-17A, and TNFα.  

      Standard Info Sheet: Indicates reconstitute volume and standard concentrations.

Basic Kit for Premixed Panels: 

• 1x Streptavidin-PE (SAPE). 

  • 96 Tests PN: 07SP0011. One bottle containing 2.5 mL of 1x SAPE.

  • 32 Tests PN: 07SP0011T. One bottle containing 1 mL of 1x SAPE.

• 10x Reading Buffer. 

  • 96 Tests PN: 07RB1. One bottle containing 4 mL of 10x buffer.

  • 32 Tests PN: 07RB1T. One bottle containing 1.5 mL of 10x buffer.

• 10x Wash Buffer. 

  • 96 Tests PN: 07WB1. One vial containing 15 mL of 10x buffer.

  • 32 Tests PN: 07WB1T. One vial containing 5 mL of 10x buffer.

• PCR 8-tube Strip. 

  • 96 & 32 Tests PN: 07P8T1. 2 strips.

• Filter Plate and Lid. 

  • 96 & 32 Tests PN: 07FPL1. 1 96- well filter plate and 1 plate lid.

• Plate Seals. 

  • PN: 07PSR1. 96 Tests: 4 sealers. 32 Tests: 3 sealers.

Application: Optimal antibody pair and antigen standard for assaying Rat Th1/Th2/Th17 7-Plex Panel. Can be multiplexed with other analytes in Rat Group 1. To be used in conjunction with the AimPlex Mouse/Rat Basic Kit Premixed Panels (PN: U100005), 32 Tests (PN: U100005T) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.

Storage:  2-8 C in the dark.

Important: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

Assay Specifications:

• Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

• Sensitivity (LOD, pg/mL)   LLOQ: pg/mL   ULOQ pg/mL

• Rat IFNg         <2                     <5                   > 5,000

• Rat IL-2          <2                     <5                   > 5,000

• Rat IL-4          <2                     <5                   > 5,000

• Rat IL-6          <2                     <5                   > 5,000

• Rat IL-10         <2                     <5                   > 5,000

• Rat IL-17A       <2                     <5                   > 5,000

• Rat TNFa       <2                     <5                   > 5,000

• Standard dose recovery: 70-130%

• Intra-assay CV: < 10%

• Inter-assay CV: < 20%

• Cross-reactivity of analytes in Rat Group 1: Negligible

• Sample volume: 15 µL/test

 For Research Use Only.  Not for use in diagnostic procedures.