PN: C191014

COMPONENTS:

1x Ab-conjugated beads (premixed). PN: C191014A. One bottle containing 5 mL of the following AimPlex antibody-bead conjugates:

       S4P2 - anti-H GM-CSF

       S4P3 - anti-H TNFα

       S4P7 - anti-H IL-2

       S4P9 - anti-H IFNγ

       S4P10 - anti-H IL-9

       S5P2  - anti-H IL-12p70

       S5P4 - anti-H IL-4

       S5P5 - anti-H IL-5

       S5P6 - anti-H IL-6

       S5P7 - anti-H IL-17A

       S5P8 - anti-H IL-22

       S5P9 - anti-H IL-21

       S5P10 - anti-H IL-10

       S5P12 - anti-H IL-13

2x Biotin-detection Ab (premixed). PN: C191014B. One vial containing 1.5 mL of the following biotinylated antibodies.

       Biotin - anti-H GM-CSF

       Biotin - anti-H TNFα

       Biotin - anti-H IL-2

       Biotin - anti-H IFNγ

       Biotin - anti-H IL-9

       Biotin - anti-H IL-12p70

       Biotin - anti-H IL-4

       Biotin - anti-H IL-5

       Biotin - anti-H IL-6

       Biotin - anti-H IL-17A

       Biotin - anti-H IL-22

       Biotin - anti-H IL-21

       Biotin - anti-H IL-10

       Biotin - anti-H IL-13

2x NR dAb Diluent. PN: 07NDX21. One vial containing 1.5 mL of biotin detection antibody diluent.

Lyophilized Standard Mix- Human T Helper Cytokine 14-Plex. PN: HG10014. One vial containing lyophilized recombinant GM-CSF, IFNγ, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-21, IL-22  and TNFα

      Standard Info Sheet: Indicates reconstitute volume and standard concentrations.

Basic Kit for Premixed Panels: 

      1x Streptavidin-PE (SAPE). PN: 07SP0011. One bottle containing 3 mL of 1x SAPE.

      10x Reading Buffer. PN: 07RB1. One bottle containing 5 mL of 10x buffer.

      10x Wash Buffer. PN: 07WB1. One vial containing 15 mL of 10x buffer.

      PCR 8-tube Strip. PN: 07P8T1. 2 strips.

      Filter Plate and Lid. PN: 07FPL1. 1 96- well filter plate and 1 plate lid.

      Plate Seals. PN: 07PSR1. 6 sealers.

STORAGE:  2-8 C in the dark.

IMPORTANT: Sodium azide forms explosive compounds with heavy metals. These products contain <0.05% (w/w) azide which with repeated contact with lead and copper commonly found in plumbing drains may result in the buildup of shock sensitive compounds. Dispose in accordance with regulations from your institute.

APPLICATION: Optimal antibody pairs and antigen standards for assaying the human T Helper Cytokine 14-Plex Panel.  To be used in conjunction with the AimPlex Basic Kit for Premixed Panels - 96 Tests (PN: U100005) and a diluent kit. Refer to the AimPlex Multiplex Immunoassay User Manual and kit inserts for the assay procedure.   

Assay Specifications: 

Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate

Sensitivity (LODs): GM-CSF, < 5 pg/mLIFNγ, < 3 pg/mLIL-2, < 3 pg/mLIL-4, < 1 pg/mLIL-5, < 2 pg/mLIL-6, < 5 pg/mLIL-9, < 5 pg/mL; IL-10, < 2 pg/mL; IL-12p70, < 3 pg/mL; IL-13, < 5 pg/mL; IL-17A, < 3 pg/mL; IL-21, < 5 pg/mLIL-22, < 3 pg/mLTNFα, < 1 pg/mL.

LLOQs: GM-CSF, < 10 pg/mLIFNγ, < 10 pg/mL; IL-2, < 10 pg/mLIL-4, < 3 pg/mLIL-5, < 5 pg/mL; IL-6, < 10 pg/mLIL-9, < 10 pg/mL; IL-10, < 5 pg/mL;  IL-12p70, < 10 pg/mL; IL-13, < 10 pg/mL; IL-17A, < 10 pg/mLIL-21, < 10 pg/mLIL-22, < 10 pg/mL; TNFα, < 2 pg/mL.

ULOQs: GM-CSF,  > 5,000 pg/mL; IFNγ, > 2,000 pg/mL; IL-2, > 2,000 pg/mL; IL-4, > 1,000 pg/mLIL-5, > 2,000 pg/mL; IL-6, > 5,000 pg/mL; IL-9, > 5,000 pg/mL; IL-10, > 5,000 pg/mL; IL-12p70, > 2,000 pg/mL;  IL-13, > 5,000 pg/mL; IL-17A, > 1,000 pg/mLIL-21, > 5,000 pg/mLIL-22, > 2,000 pg/mLTNFα, > 1,000 pg/mL.

Standard dose recovery: 70-130%

Intra-assay CV: < 10%

Inter-assay CV: < 20%

Cross-reactivity of analytes in the Panel: Negligible

Sample volume: 15 µL/test

For Research Use Only.  Not for use in diagnostic procedures.